Cell Respiration in Pea Seeds
Background:
Cellular respiration is the process that allows organisms to convert energy obtained from eating food into usable energy in the form of ATP. During Respiration, Oxygen and Glucose are the products of the reaction and Carbon Dioxide, water, and ATP are the products. Cellular respiration can be measured with a respirometer. As oxygen is consumed during respiration, water will enter the respirometer due to the decrease in the volume. KOH is added to repirometers in order to convert the Carbon dioxide released from respiration into small carbon atoms so that the volume can decrease.
Purpose: The purpose of this experiment is to determine the respiration rate of germinated pea seeds in comparison to dormant pea seeds.
Hypothesis: The Germinated peas will have a higher respiration rate than the non germinated peas because the germinated peas require oxygen for cell respiration and the dormant ones do not.
Materials:
- 25 germinated pea seeds
- 25 dormant pea seeds
- 3 vials
- 3 rubber stoppers
- 3 pipets
- cotton
- KOH
- water bin
- masking tape
- graduated cylander
Building a Respirometer
1. Place 3 drops of Koh on a piece of cotton.
2. place the piece of cotton on the bottom of the vial.
3. Place another piece of cotton in the vial on top of the first one.
4. place the peas in the vial.
5. seal the vial with the rubber stopper.
6. place the pipet in the rubber stopper. It may help to add tape to the outside of the pipet to achieve a more airtight seal.
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Iv: material in the respiometer
Dv: amount the operas respire
Control group: respiromerer with glass beads
Constants: volume in RESPIROMETers, temperature, amount of peas
Results:
Reading of Respirator
Time
Test Group
Germinated Pea Seeds
Dormant Pea Seeds
Glass beads
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0 Minutes
0.9
0.9
0.9
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5 Minutes
0.79
0.89
0.89
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10 Minutes
0.7
0.88
0.89
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